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The second response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circular dichroism. As drawn in Fig - https://pagespeed.web.dev/analysis/https-olisclarity-com/ft59obxjpp?form_factor=mobile. 9, the reaction might also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As displayed in Fig. 9, this might be since the H4B radical acts as an electron sink in a later point in the response, so as to permit NOS to, Fig. 9. A model for Web Site NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.

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